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Revvity
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BMG Labtech
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SLM Aminco Instruments Inc
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MultiCell Technologies
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JEOL
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Image Search Results
Journal: Frontiers in Cell and Developmental Biology
Article Title: Additive Antiproliferative and Antiangiogenic Effects of Metformin and Pemetrexed in a Non-Small-Cell Lung Cancer Xenograft Model
doi: 10.3389/fcell.2021.688062
Figure Lengend Snippet: The combination of metformin with pemetrexed exerted additive inhibitory effects on A549 cell growth in a human orthotopic NSCLC xenograft model. An orthotopic lung tumor model was established via transpleural injection of 1 × 10 6 fluorescently labeled A549 cells (iRFP-2A-Venus). After 4 weeks of A549 grafting, the mice were treated with metformin (Met; 250 mg/kg/day), pemetrexed (Pem; 150 mg/kg/twice a week) or both (Met + Pem). [ (A) left panel] After a 3-week period of drug treatment, in vivo fluorescence images were acquired using an IVIS ® (Spectrum CT instrument) at the appropriate wavelength (iRFP: λ 650/720 nm). [ (A) right panel] Statistical bar chart shows the total radiant efficiency of the iRFP signals, which was calculated using Living Image software. [ (B) left panel] Whole lungs were excised from nude mice, and ex vivo fluorescence imaging was observed directly through the IVIS Spectrum system. [ (B) right panel] Quantitative analysis of fluorescence intensity based on ex vivo images. All images were scaled to the same minimum and maxi-mum color values. The data are presented as the mean ± SD of those from 2–3 independent experiments with two technical replicates in each. * P < 0.05, ** P < 0.01, and *** P < 0.001.
Article Snippet: On week 3, after drug treatment, the iRFP fluorescence was detected by an
Techniques: Injection, Labeling, In Vivo, Fluorescence, Software, Ex Vivo, Imaging